Please consider the following points to help formulate your research question and outline a search strategy.
Excerpted and modified from: "AWIC Tips for Searching for Alternatives to Animal Research and Testing".
General example as to avoid unnecessary duplication of already published research:
In (Animal Model), what is the effect of (Intervention/Exposure) in achieving (Outcome) related to (Disease/Condition)?
Pain and Stress Prevention:
Is (Administration Route A) or (Administration Route B) more effective at eliminating unnecessary pain or stress in (Animal Model) with (Disease/Condition)?